The process The IndiTreat® test starts with the extraction of a patient’s tumor specimen through any of the standard methods. Fresh specimens (living cells) are shipped immediately in proprietary transportation containers to the specialized laboratory. Carefully processed and transferred into appropriate culture media at our laboratory, live tumor cell clusters grow and multiply under controlled conditions to form hundreds of sibling 3D-microtumors, known as tumoroids, which have been shown to preserve the colorectal adenocarcinomic histology and protein expression patterns of their original tumor2. The innovative approach is now to convert a tumor’s most harmful property – growth – into actionable diagnostic information – growth inhibition. This is achieved by better understanding the growth dynamics of metastatic tissue and its drug sensitivity in terms of growth behavior. For the actual drug sensitivity test phase, hundreds of cultivated tumoroids are precisely seeded into multiwell plates. These controlled environments contain defined concentrations of the antineoplastic drugs and drug combinations of interest. Growth inhibition is the key The tumoroids’ further growth, steady state or decline in the presence or the absence of the drug compounds in question is their biological response. Growth patterns are analyzed using imageanalysis and proprietary AI algorithms are converted into a “relative growth inhibition” score. Each patient sample is tested in multiple replicates and the process includes a positive and a negative control. The scores are compared to those of respective How it works. The process underpinning IndiTreat® Results from large cancer surveys show that the majority of deaths from solid tumors are caused by metastases1. It is the underlying spread of the tumor and metastatic growth that poses the vital threat.
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