1. Informed Consent

The doctor informs the patient about the benefits and risks of the IndiTreat® test. When agreement to perform the IndiTreat® test is achieved, the Test-Order form can be signed.

2. Tissue sampling and shipping

A tumor sample is collected from a resected specimen or during a biopsy typically from a metastasis in the liver. The tumor tissue is placed in a special medium to keep the tumor cells alive and transported in a dedicated 2cureX transport box to a nearby 2cureX laboratory within 24 hours

3. Processing the tumor sample

In the first part of the IndiTreat® test, the 2cureX laboratory creates hundreds to thousands of 3D microtumors from the collected tumor tissue. Subsequently, the microtumors are grown in tubes of the laboratory.

4. Conducting IndiTreat® test

After some days of growth, the 3D microtumors are treated with a panel of different cancer drugs or drug combinations in order to measure disintegration (death) of the microtumors, inhibition of growth or continuous growth.

5. Evaluation and reporting

The response of microtumors to the different drugs is classified as drug sensitive or drug resistant. An IndiTreat test report is send to the doctor.

6. Individualized therapy choice

Based on the IndiTreat® result and after consideration of all available information on the patient, the doctor decides which treatment she/he will recommend the patient.

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